Composite
Part:BBa_K3081004
Designed by: Xiaoying Qiao Group: iGEM19_Peking (2019-10-19)
pTAC GFP
Tac promoter is a hybrid between trp and lacUV5 promoters. Tac promoter is a kind of strong E. coli promoter and is IPTG inducible. pTAC promoter induces the protein dCas9,and the C terminus of dCas9 is fused to GFP coding sequence. Expression level of dCas9 induced by IPTG is tested by fluorescence of fused dCas9 linker GFP
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1472
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Categories
Parameters
None |