Composite

Part:BBa_K3081004

Designed by: Xiaoying Qiao   Group: iGEM19_Peking   (2019-10-19)
Revision as of 17:18, 19 October 2019 by Registry (Talk | contribs)

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pTAC GFP

Tac promoter is a hybrid between trp and lacUV5 promoters. Tac promoter is a kind of strong E. coli promoter and is IPTG inducible. pTAC promoter induces the protein dCas9,and the C terminus of dCas9 is fused to GFP coding sequence. Expression level of dCas9 induced by IPTG is tested by fluorescence of fused dCas9 linker GFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1472


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