Part:BBa_K3286215

Two component system RpfCch + RpfG The folowwing composite parts contains the cyanobacterial circadian oscillator core genes. The core circadian clock of the cyanobacterium Synechococcus elongatus PCC 7942 is composed of three components, KaiA, KaiB, and KaiC, expressed as monocistronic kaiA and dicistronic kaiBC transcripts [1]. The kaiBC operon is upregulated by the KaiA protein and downregulated by the KaiC protein to generate a circadian oscillator. KaiB can bind to KaiA, therefore opposing KaiA’s stimulatory activity by sequestering it, and to KaiC, driving the KaiC phosphorylation cycle in a clockwise direction.

1. [1]Cohen, SE., and Golden, SS. (2015) Circadian Rhythms in Cyanobacteria. Microbiol. Biol. Rev. 79(4), 373-385.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1922
  Illegal PstI site found at 887
  Illegal PstI site found at 1493
  Illegal PstI site found at 2120
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1922
  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal PstI site found at 887
  Illegal PstI site found at 1493
  Illegal PstI site found at 2120
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1922
  Illegal BglII site found at 288
  Illegal BglII site found at 3362
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1922
  Illegal PstI site found at 887
  Illegal PstI site found at 1493
  Illegal PstI site found at 2120
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1922
  Illegal PstI site found at 887
  Illegal PstI site found at 1493
  Illegal PstI site found at 2120
  Illegal NgoMIV site found at 818
  Illegal NgoMIV site found at 909
  Illegal NgoMIV site found at 1736
  Illegal NgoMIV site found at 1831
  Illegal NgoMIV site found at 2890
  
• 1000
  COMPATIBLE WITH RFC[1000]