Composite
Part:BBa_K3152019
Designed by: Xixi Song Group: iGEM19_ASTWS-China (2019-10-17)
Revision as of 17:44, 19 October 2019 by LucyShi 2018 (Talk | contribs)
mecI-mec operator-mCherry
This part contains “Anderson Medium-RBS-mecI-mec operator-RBS-mCherry” (BBa_K3152004 ,BBa_K3152005).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 379
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Then we used pSB1C3-GFP as the backbone and instead GFP of mec operator-mCherry (BBa_K3152005). Then the mecI pathway (BBa_K3152004 ) was added into the plasmid pSB1C3-mec-mCherry. As shown in Figure1 below, bacteria colonies did not present the green fluorescence when our DNA fragments were added to the whole plasmid. Also, because of the present of mecI repressor, the color of mCherry disappeared. Three colonies were chosen from the medium and the plasmids were extracted and verified by digesting with restriction enzyme EcoRI and PstI.
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Categories
Parameters
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