Part:BBa_K3031015
LuxI with FUR box located in the -10 region
This is the LuxI promoter sequence with a standard Ferric Uptake Regulator sequence (FUR box) inserted between the -35 and -10 region. The FUR box allows for the repression of Quroum Sensing expression of genes associated in the presence of ferric iron. In the classical FUR repression mechanism, iron-bound Fur binds to a Fur box sequence that overlaps with, or is proximal to, promoters of iron responsive genes, thus preventing their transcription. When intracellular iron is depleted, Fe2+ is released from Fur, causing conformational changes in the protein resulting in dissociation from the Fur box.
In a low iron environment, through the regulation of the LuxI-Fur box promoter, the LuxI protein (AHL) would be produced as normal which forms an integral part of the well characterized and used LuxR/LuxI quorum sensing system.
In the absence of Fe the downstream genes are once gain turned on at high cell densities.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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