Regulatory

Part:BBa_K678004

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-04)
Revision as of 11:04, 17 October 2019 by Helen M (Talk | contribs)


PGK, mammalian promoter

Promoter for expression of genes in mammalian cells

Contribution: NUDT_CHINA 2019

Summary：in this part, we made contributions to the quantitative characterization data of mammalian PGK Promoter. We measured the strength of this promoter by expression change of firefly luciferase at continous time set after transfection of 6, 18, 30, 42 and 54 hours in HepG2 cell line, and made a curve showing how the strength of PGK promoter changes within 48 hours.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 143
    Illegal PstI site found at 400
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 143
    Illegal PstI site found at 400
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 143
    Illegal PstI site found at 400
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 143
    Illegal PstI site found at 400
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/human
//direction/forward
//promoter
Parameters
None