Composite

Part:BBa_K3031019:Design

Designed by: David Doyle   Group: iGEM19_SUIS_Shanghai   (2019-10-15)
Revision as of 00:50, 16 October 2019 by Registry (Talk | contribs)

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Constitutive promoter + OxyR + TrxC promoter + GFP generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 781
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734
    Illegal BsaI.rc site found at 1894


Design Notes

Mutations of the OxyR protein.


Source

Yang Ming University iGEM team 2013

OxyR sequence from Uniprot and then sequenced by Genscript.

References