Composite

Part:BBa_K3040007

Designed by: TSAI, CHIA-TSEN   Group: iGEM19_NTHU_Taiwan   (2019-10-18)
Revision as of 13:54, 18 October 2019 by Registry (Talk | contribs)

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placUV5-R0010-TesA_pFadBA- RBS -mRFP

Tes A is a heterologous thioesterase, which is able to hydrolyze fatty acyl-CoAs to free fatty acids. Here, we combine Tes A with pFadBA (wild type) together as a new promoter, Tes A pFadBA, to see if the sensitivity range of fatty acid concentration lowers due to the increase of endogenous fatty acid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1106
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1107
    Illegal PstI site found at 1121
    Illegal NotI site found at 1114
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1172
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1107
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1107
    Illegal PstI site found at 1121
    Illegal AgeI site found at 1829
    Illegal AgeI site found at 1941
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None