Coding

Part:BBa_K3187028

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-12)
Revision as of 01:49, 21 October 2019 by Jaminersss (Talk | contribs)


Sortase A7M (Ca2+-independent variant)

Profile

Name Sortase A7M
Base pairs 470
Molecular weight 17.85 kDa
Origin Staphylococcus aureus, synthetic
Parts Basic part
Properties Ca2+-independent, transpeptidase, linking sorting motif LPXTG to poly-glycine Tag

Usage and Biology

Methods

Results

References

  1. ↑ Hee-Jin Jeong, Gita C. Abhiraman, Craig M. Story, Jessica R. Ingram, Stephanie K. Dougan, Generation of Ca2+-independent sortase A mutants with enhanced activity for protein and cell surface labeling, PLOS ONE, 2017 [1]
  2. ↑ Hongyuan Mao, Scott A. Hart, Amy Schink, and Brian A. Pollok, Sortase-Mediated Protein Ligation: A New Method for Protein Engineering, JACS, 2004 [2]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part/winner
Parameters
None