Coding

Part:BBa_K2449003

Designed by: Malte Skovsager Andersen   Group: iGEM17_SDU-Denmark   (2017-07-20)
Revision as of 15:36, 16 October 2019 by Willie Jin (Talk | contribs)


cep94A

This is the so called cellobiose phosphorylase (Cep94A), which phosphorylates the cellobiose β-linkage resulting in its breakdown to D-glucose and α-D-glucose-1-phosphate. It is used in the following biobrick BBa_K2449004 For more info visit https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294459/

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 846
    Illegal NgoMIV site found at 1942
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 108


ECUST_China 2019 characterization

In ECUST_China 2019 characterization, we used Cep 94A to degrade cellobiose to glucose and glucose-phosphate. To characterize the Cep 94A specifically, we performed SDS-PAGE and measured enzyme activity.

SDS-PAGE revealed that Cep 94A was expressed successfully (about 91kDa). After that, we sonicated E.coli and extract Cep 94A, using DNS assay to measure the enzyme activity.

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