Coding

Part:BBa_K2960010:Design

Designed by: Ann Metzloff   Group: iGEM19_Cornell   (2019-10-16)
Revision as of 01:30, 17 October 2019 by Aemetzloff (Talk | contribs) (→‎Design Notes)


The mlrE enzyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1125
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 84
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mlrE gene sequence has been codon-optimized for expression in E. coli.

The construct was designed to be RFC[10] compatible.

Source

Sphingopyxis sp. genomic sequence. Jin H, Nishizawa T, Guo Y, Nishizawa A, Park H-D, Kato H, Tsuji K, Harada K-I. 2018. Complete genome sequence of a microcystin-degrading bacterium, Sphingosinicella microcystinivorans strain B-9. Microbiol Resour Announc 7:e00898-18. https://doi.org/10.1128/MRA.00898-18.

References