Coding

Part:BBa_K2960003:Design

Designed by: Michelle Croen   Group: iGEM19_Cornell   (2019-10-16)
Revision as of 01:07, 21 October 2019 by Aemetzloff (Talk | contribs) (→‎References)


mlrC with TorA tat Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 722
    Illegal BglII site found at 932
    Illegal BamHI site found at 1467
    Illegal XhoI site found at 1211
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was designed to be RFC[10] compatible.


Source

Sphingopyxis sp. C-1 genomic sequence. European Nucleotide Archive. (2008 October 22). Received from https://www.ebi.ac.uk/ena/data/view/AB468060

References

Lee, P. A., Tullman-Ercek, D., & Georgiou, G. (2006). The bacterial twin-arginine translocation pathway. Annual review of microbiology, 60, 373–395. doi:10.1146/annurev.micro.60.080805.142212