Composite

Part:BBa_K3081044

Designed by: Le Zhang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 18:10, 19 October 2019 by Xiaoying (Talk | contribs)


pBAD-dCas9-ssrA-J23119-NT1-J23119-mRFP

This part is an improvement of BBa_K3081024, which we add a degradation signal peptide ssrA to the dCas9. A more gentle decrease in fluorescence is observed when dCas9 is fused to ssrA tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2321
    Illegal NheI site found at 5495
    Illegal NheI site found at 5518
    Illegal NheI site found at 5626
    Illegal NheI site found at 5649
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 4600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 6209
    Illegal AgeI site found at 6321
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None