Coding
Part:BBa_K3320013
Designed by: Joseana Mendes Comodaro Group: iGEM19_Amazonas-Brazil (2019-10-20)
Revision as of 21:29, 20 October 2019 by Jocomodaro (Talk | contribs)
GFPmut3 + HlyA
This is GFPmut3 based on BBa_E0040 with a secretion tag from the alpha-hemolysin system.
Usage and Biology
GFP is a fluorescent protein from Aequorea victoria. By adding the HlyA sequence to the C-terminus of the gene, this allows the fusion of this signal peptide to the GFP, enabling its secretion directly from the cytosol into the extracellular environment.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |