Coding

Part:BBa_K2960008

Designed by: Ann Metzloff   Group: iGEM19_Cornell   (2019-10-16)
Revision as of 00:36, 17 October 2019 by Registry (Talk | contribs)

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The mlrC metalloprotease

A cassette of enzymes endogenous to Sphingopyxis sp. has been shown to sequentially break down microcystin-LR (the most common and toxic microcystin that results from harmful algal blooms). The enzymes have been named mlrA, mlrB, mlrC, mlrD, mlrE, and mlrF.

MlrC is the third enzyme in the microcystin-degradation pathway. MlrC is assumed to be a metallopeptidase. It cleaves the tetrapeptide intermediate into an Adda amino acid and other smaller peptides. The exact smaller peptides that result are still unknown.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 549
    Illegal BglII site found at 759
    Illegal BamHI site found at 1294
    Illegal XhoI site found at 1038
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None