Coding

Part:BBa_K3286200

Designed by: Ben Kuipers   Group: iGEM19_Wageningen_UR   (2019-10-19)
Revision as of 08:03, 20 October 2019 by Benkuipers (Talk | contribs)


RpfCch

The RpfCch is a chimeric protein consisting of the RpfC X. fastidiosa N-terminal transmembrane domain (TMD) and the RpfC C-terminal histidine kinase part from X. campestris. This protein has been proven to sense X. fastidiosa DSF, as with the native X. fastidiosa RpfC, but now the requirement of another protein (X. fastidiosa RpfF) is not needed to sense DSF. The DSF sensing protein RpfC of X. campestris is already present in the repository under BBa_K1315003.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 826
    Illegal PstI site found at 1432
    Illegal PstI site found at 2059
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 826
    Illegal PstI site found at 1432
    Illegal PstI site found at 2059
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1861
    Illegal BglII site found at 227
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 826
    Illegal PstI site found at 1432
    Illegal PstI site found at 2059
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 826
    Illegal PstI site found at 1432
    Illegal PstI site found at 2059
    Illegal NgoMIV site found at 757
    Illegal NgoMIV site found at 848
    Illegal NgoMIV site found at 1675
    Illegal NgoMIV site found at 1770
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None