Device

Part:BBa_K2936019

Designed by: Weng Jiaqi   Group: iGEM19_ZJUT-China   (2019-10-10)
Revision as of 11:23, 10 October 2019 by Registry (Talk | contribs)

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pBAD-lacZ alpha-pFrmR-AraC-CAT

The AraC protein would binds to AraBAD promoter, inhibiting its expression. At this time, only CAT gene is expressed in the pathway, then the culture added with TMB will turns blue. And when the pFrmR turn off, without repressor protein the AraBAD promoter activate, the LacZɑ expresses and the culture with ONPG would turns orange.Those parts form a bidirectional switch determined by formaldehyde concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3178
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3178
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 120
    Illegal BamHI site found at 182
    Illegal BamHI site found at 2421
    Illegal XhoI site found at 3214
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3178
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3178
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None