Device
Part:BBa_K2936019
Designed by: Weng Jiaqi Group: iGEM19_ZJUT-China (2019-10-10)
pBAD-lacZ alpha-pFrmR-AraC-CAT
The AraC protein would binds to AraBAD promoter, inhibiting its expression. At this time, only CAT gene is expressed in the pathway, then the culture added with TMB will turns blue. And when the pFrmR turn off, without repressor protein the AraBAD promoter activate, the LacZɑ expresses and the culture with ONPG would turns orange.Those parts form a bidirectional switch determined by formaldehyde concentration.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3178
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3178
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 120
Illegal BamHI site found at 182
Illegal BamHI site found at 2421
Illegal XhoI site found at 3214 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3178
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3178
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |