Device

Part:BBa_K2940014

Designed by: Jakub Chromy   Group: iGEM19_Edinburgh_OG   (2019-10-16)
Revision as of 16:57, 16 October 2019 by Registry (Talk | contribs)

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XylR coding device-sfGFP_optimism coding device with Pu

This part encodes the biosensor circuit comprising the toluene-specific transcription factor XylR and the sfGFP reporter gene. The part was meant to serve as the starting biosensor circuit platform for the subsequent directed evolution of XylR in order to develop a biosensor for the detection of aromatic amines.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2101
    Illegal XhoI site found at 3018
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/gfp
//cds/transcriptionalregulator/activator
//function/reporter/fluorescence
Parameters
chassisEscherichia coli TOP10
device_typeBiosensor
emission509 nm
excitation490 nm
proteinsXylR BBa_K2023005, sfGFP BBa_K2541400