Device

Part:BBa_K2940014:Design

Designed by: Jakub Chromy   Group: iGEM19_Edinburgh_OG   (2019-10-16)
Revision as of 20:54, 20 October 2019 by Jakub Chromy (Talk | contribs) (→‎Source)


XylR coding device-sfGFP_optimism coding device with Pu


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2101
    Illegal XhoI site found at 3018
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is identical to BBa_K2023015 part developed by Ionis Paris iGEM team 2016 [1], except for the originally used wild-type GFP (BBa_E0040) reporter gene being replaced by codon-optimized superfolder GFP (BBa_K2541400).

The codon-optimized superfolder GFP was used as the reporter in an attempt to have a very sensitive starting biosensor genetic circuit for the subsequent directed evolution and screenings for the biosensors with new substrate specificities.

Source

References

[1] http://2016.igem.org/Team:Ionis_Paris