Coding

Part:BBa_K2924021:Design

Designed by: Arian Abbasi   Group: iGEM19_Duesseldorf   (2019-10-13)
Revision as of 19:48, 20 October 2019 by Mickgottemeier (Talk | contribs)


accABCD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 668
    Illegal AgeI site found at 1501
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 606
    Illegal SapI.rc site found at 803


Design Notes

The genes from E. Coli K12</> were cloned into pet21a in an operon-like manner to ensure transcription of all genes of the complex at the same level and from the same vector.


Source

The genes were cloned from the genome of <i>E. coli K12