Composite
Part:BBa_K3081041
Designed by: Song-Yuan Zhang Group: iGEM19_Peking (2019-10-16)
CRISPR-based replication interference system for p15A plasmid copy number control, ini(-) site
dCas9 based replication control on p15A origin plasmid copy number——bottom strand of initiation site.This part is an improvement ofBBa_K3081018, which we add a degradation signal peptide ssrA to the dCas9.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 2321
Illegal NheI site found at 5495
Illegal NheI site found at 5518 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 4600 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 5522
Illegal AgeI site found at 979 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
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Categories
Parameters
None |