Project

Part:BBa_K3113302

Designed by: Alejandro Salinas Illarena   Group: iGEM19_Munich   (2019-10-12)
Revision as of 18:53, 20 October 2019 by Theresakeil (Talk | contribs)


pCAG_Gag-HiBiT-L7Ae

This plasmid codes for the coat protein of gag fused to a split luciferase fused to an RNA binding protein. This construct forms virus-like particles which can be detected via a split luciferase assay. Through the RNA binding protein, the vesicles can be loaded with RNA.


Usage

pCAG_GAG-HiBit-L7Ae construct is a Composite part that has been designed to achieve VLP formation inside HEK293T/MIN6-K8 cells and enable the transport of chosen RNA information inside vesicles through L7Ae-C/D box interaction (together with construct BBa_K3113301[1] and BBa_K3113203[2]).

Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3019
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2319


  1. ↑ https://parts.igem.org/Part:BBa_K3113301
  2. ↑ https://parts.igem.org/Part:BBa_K3113303
[edit]
Categories
//awards/composite_part/nominee
Parameters
None