Regulatory
Part:BBa_K3138000
Designed by: Kamila Liman, Jagoda Pierścińska Group: iGEM19_Wroclaw (2019-10-15)
Metallothionein-IV promoter Uniprot Q9HFC9-1
Part contains the promoter sequence that in native cells activates the metallothionein IV (MTP IV) transcription after the cell exposure to high concentrations of heavy metals (for example Cu, Pb, Cd). Thus, the level of protein expression is regulated by the concentration of metal ions in the medium.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 95
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 95
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 95
Illegal BglII site found at 380
Illegal BglII site found at 492 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 95
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 95
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//promoter
biology | Yarrowia lipolityca E150 |
direction | Forward |
positive_regulators | 1 |