Regulatory

Part:BBa_K3138000

Designed by: Kamila Liman, Jagoda Pierścińska   Group: iGEM19_Wroclaw   (2019-10-15)
Revision as of 23:02, 15 October 2019 by Registry (Talk | contribs)

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Metallothionein-IV promoter Uniprot Q9HFC9-1

Part contains the promoter sequence that in native cells activates the metallothionein IV (MTP IV) transcription after the cell exposure to high concentrations of heavy metals (for example Cu, Pb, Cd). Thus, the level of protein expression is regulated by the concentration of metal ions in the medium.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 95
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 95
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 95
    Illegal BglII site found at 380
    Illegal BglII site found at 492
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 95
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 95
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
biologyYarrowia lipolityca E150
directionForward
positive_regulators1