DNA

Part:BBa_K3171177

Designed by: Yashika Venkatesh   Group: iGEM19_Groningen   (2019-10-19)
Revision as of 17:51, 20 October 2019 by Yashikavenkatesh (Talk | contribs)


HisD 3000bp Homologous part 1

We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. To make a histidine auxotroph by knocking out HisD in E. coli specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts (BBa_K3171177, BBa_K3171178, BBa_K3171179) in order to decide on the correct homologous part. 3 gRNA target sites (BBa_K3171180, BBa_K3171181, BBa_K3171182) were selected for knock out which.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 689
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1472
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 689
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 689
    Illegal AgeI site found at 1885
    Illegal AgeI site found at 2788
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 807


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Categories
Parameters
None