Composite

Part:BBa_K3257104

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-10-08)
Revision as of 10:41, 8 October 2019 by Registry (Talk | contribs)

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Cre Recombinase with SsrA(LVA)-aTc Inducible

According to our modeling, the number of Cre recombinase in one E.coli cell should be a relatively small one, more specifically 27 per cell, so that mutations will accumulate after rounds of R-Evolution process. Based on this instruction, we have spent much effort in lowering the steady-state expression level of Cre. An important approach is to attach 5 different types of degradation tags after the CDS of Cre. Using EGFP as a reporter, we can see that degradation tags lead to fluorescence reduction.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 436
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 122
  • 1000
    COMPATIBLE WITH RFC[1000]


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