Composite

Part:BBa_K3286104

Designed by: Robert Hooftman   Group: iGEM19_Wageningen_UR   (2019-10-14)
Revision as of 16:34, 14 October 2019 by Registry (Talk | contribs)

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Expression cassette for small proteins in E. coli BL21DE3

Use this part, containing a T7 promoter, BCD system, Strep tag, MBP and TEV site to produce small protein domains in high volumes. After isolation of the fusion protein and treatment with TEV protease, run it again over a strep column to remove the Maltose binding protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 493
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 191


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Categories
Parameters
None