Measurement

Part:BBa_K3286046

Designed by: Sebastiaan Kuiper   Group: iGEM19_Wageningen_UR   (2019-10-16)
Revision as of 16:05, 20 October 2019 by SJK (Talk | contribs)


mRFP-GFP divergent, Bacteriophage Lambda promoters, operators and 5'UTR's

Monomeric Red fluorescent protein (mRFP) part:BBa_K3286045 and Green fluorescent protein (GFP) BBa_E0040 downstream of bacteriophage lambda PL, PRM and PR promoter regions (including the operators OL and OR, and the 5'UTR's) part:BBa_K3286045. This part has been used to asses the potential of bacteriophage lambda inhibition by CRISPRi, by targeting essential early promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7
    Illegal AgeI site found at 119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1723


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Categories
Parameters
None