Regulatory

Part:BBa_K3171173:Design

Designed by: Yashika Venkatesh   Group: iGEM19_Groningen   (2019-10-16)
Revision as of 13:34, 20 October 2019 by Yashikavenkatesh (Talk | contribs) (→‎Design Notes)


Improved pTet promoter in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to enhance function and inducibility. We optimized tetracycline-inducibility in E. coli by creating randomizations in the sequence to obtain arbitrary promoters. The screening was done based on quantifications of the fluorescence intensity measured. The best version of the promoter was determined based on fold change in the fluorescence intensity and low basal level. This promoter is also used in the composite part with mCherry fusion protein.

Source

pTET was obtained from the Biobrick registry BBa_R0040

References