Part:BBa_K2924006

guideRNA from acetyl coenzyme A acetyltransferase

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Usage and Biology

Fig. 1: Position of sgRNA (orange) in the beta ketoacyl-acyl carrier protein synthase gene.

Fig. 2: Reaction catalyzed by ketoacyl-acyl carrier protein synthase.

https://parts.igem.org/File:Thiolase_gene_location.png https://parts.igem.org/File:Thiolase_reaction.png https://parts.igem.org/File:Knock_in_Schema.png https://parts.igem.org/File:CRISPR_dCas9.png

Fig. 3: Scheme of a knock-in as a consequence of homologous recombination in Synechocystis.

Fig. 4: Scheme of function of the CRISPRi/dCas9 - system. The dCas9 (yellow) binds with the sgRNA to the complementary DNA strand and inhibits the transcription by RNA polymerase II (blue).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]