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Part:BBa_K3228002

Designed by: Maurice Mager   Group: iGEM19_Marburg   (2019-09-25)
Revision as of 10:13, 25 September 2019 by Registry (Talk | contribs)

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pMC_0_6_22_aNSo1_integration (neutral integration site for cyanobacteria)

Downstream region of the artificial neutral integration site option 1 (aNSo1). Can be used for genome integration of genes via homologous recombination in many strains of cyanobacteria such as PCC7942 and UTEX2793.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 121
    Illegal PstI site found at 232
    Illegal PstI site found at 259
    Illegal PstI site found at 289
    Illegal PstI site found at 331
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 232
    Illegal PstI site found at 259
    Illegal PstI site found at 289
    Illegal PstI site found at 331
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1019
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 121
    Illegal PstI site found at 232
    Illegal PstI site found at 259
    Illegal PstI site found at 289
    Illegal PstI site found at 331
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 121
    Illegal PstI site found at 232
    Illegal PstI site found at 259
    Illegal PstI site found at 289
    Illegal PstI site found at 331
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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