Coding

Part:BBa_K3209000

Designed by: SUN YUHAN   Group: iGEM19_JNFLS   (2019-10-15)
Revision as of 12:54, 20 October 2019 by SUNYUHAN (Talk | contribs) (The benzoylformate decarboxylase was designed to contain 7 amino acids mutations.)


Benzoylformate decarboxylase mutant (BFD-M7)

This is benzoylformate decarboxylase mutant (BFD-M7) from Pseudomonas putida. It contains 7 amino acids mutations, which sequence is different from BFD (BBa_K2155001). The mutation information of BFD-M7 is as follows: W86R, N87T, L109G, L110E, H281V, Q282F and A460M. BFD-M7 can catalyze glycolaldehyde synthesis from formaldehye, then the glycolaldehyde reacts with formaldehyde to form dihydroxyacetone (DHA), also catalyzed by this enzyme.


References:

  1. Bo Cui, Bingzhao Zhuo, Xiaoyun Lu, et al. Enzymatic synthesis of xylulose from formaldehyde. Chinese Journal of Biotechnology,2018, 34(7): 1128-1136.
  2. Xiaoyun Lu, Yuwan Liu, Yiqun Yang, et al. Constructing a synthetic pathway for acetyl

coenzyme A from one-carbon through enzyme design. Nature communications, 2019 Mar 26;10(1):1378.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 771
    Illegal NgoMIV site found at 1233
    Illegal AgeI site found at 235
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 340


[edit]
Categories
//awards/basic_part/nominee
Parameters
None