Composite

Part:BBa_K2942707:Design

Designed by: Yihui Wang   Group: iGEM19_SYSU-Medicine   (2019-10-16)
Revision as of 09:19, 16 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


CMV promoter, riboswitch,nitroreductase and eGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 901
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 901
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 901
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 901
    Illegal AgeI site found at 1456
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we optimized the nitroreductase that we carried out human codon optimization on the original nitro reductase and added flag tag for labeling, which Increases the expression of protein as well as detection .


Source

The CMV promoter+ Riboswitches+Nitroreductase+eGFP was linked into the expression vector by restriction sites EcoRI and PstI, and among these fragments we use three restrict enzyme as following: AflIII,KpnI AND PacI.

References