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Part:BBa_K3040011
Native pFadD promoter regulating downstream RFP
We create a hybrid Fatty acid/acyl-CoA-regulated promoter by the combination of pLac with a modified promoter pFadBA. It is pointed out by the previous iGEM team, UPF CRG Barcelona_2018 iGEM, that the native promoter pFadBA has great leakage and is a weak promoter that isn’t ideal enough for our system. Therefore, to enhance the sensitivity and reduce the leakage of the promoter pFadBA for making it more suitable for our system, we improved our promoter based on the previous pfadBA DNA sequence from NTU_Taida 2012. pFL1 promoter has a fadR recognition site between its -10 and -35 region, and a promoter pLac behind -10 region. pFL1 promoter is designed to respond to changes of both fatty acid and an exogenous inducer, IPTG. The hybrid promoter pFL1 is fully activated only when both the fatty acids and IPTG are present.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
Reference
ZHANG, Fuzhong; CAROTHERS, James M.; KEASLING, Jay D. Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature biotechnology, 2012, 30.4: 354.
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