Measurement

Part:BBa_K2970009

Designed by: Alexander Bast   Group: iGEM19_Hamburg   (2019-10-03)
Revision as of 12:30, 20 October 2019 by MarieTT (Talk | contribs)


J23100 Test Composition with RiboJ

Since the ribosome binding site can influence the promoter strength, we thought about an option to separate it from the promoter. For this we used the ribozyme RiboJ, which always makes the same cut between the promoter and the following sequences. Thus this plasmid can be used to measure promoters. In this case the promoter J23100 was inserted into the backbone pSB1C3 with BBa_E0240 for characterization.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 789


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