Composite
IBR-E21

Part:BBa_K3254003

Designed by: Yeqing Zong, Yuqi Pan   Group: iGEM19_GENAS_China   (2019-10-13)
Revision as of 03:33, 14 October 2019 by Registry (Talk | contribs)

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Int8attB-BsaI site-terminator-Int8attP

We expected that this part can be placed between a promoter and a translational unit part and work as a normally open (NO) switch for the downstreamed gene. and switch to ON state by excising the terminator ECK120030221 between the att sites when it reacted with Int8 integrase (BBa_K3254008). Two BsaI restriction site were added between attB site and terminator. As a result, it can work as a normally closed (NC) switch for the gene which was inserted between the two BsaI site and switch to OFF state when it was excised.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79
    Illegal BsaI.rc site found at 67


[edit]
Categories
//function/recombination
Parameters
None