Composite
IBR-G22
Part:BBa_K3254005
Designed by: Yeqing Zong, YI REN Group: iGEM19_GENAS_China (2019-10-13)
Revision as of 12:15, 20 October 2019 by Zongyeqing (Talk | contribs)
TG1attB-BsaI site-terminator-TG1attP
We expected that this part can be placed between a promoter and a translational unit part and work as a normally open (NO) switch for the downstreamed gene. and switch to ON state by excising the terminator L3S3P22 between the att sites when it reacted with TG1 integrase (BBa_K2460007). Two BsaI restriction site were added between attB site and terminator. As a result, it can work as a normally closed (NC) switch for the gene which was inserted between the two BsaI site and switch to OFF state when it was excised.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 60
Illegal BsaI.rc site found at 48
[edit]
Categories
Parameters
//function/recombination
None |