Part:BBa_K2970008
Gate/Trigger test composition
It contains both triggers and the gate to test all three. (BBa_K2970000, BBa_K2970001, BBa_K2970002). The function of the gate is the regulation of the translation, which is activated by the triggers. The two triggers form a complex due to complementary areas. The sequence that is formed is complementary to the gate that in normal conformation forms a hairpin which covers the RBS and the start codon. If the trigger complex binds to the gate, this hairpin opens and the RBS is released and translation can take place. The affinity of the trigger complex is higher than the affinity of the gate within. The gate can only be opened if both triggers are present, otherwise the complementary region is too short. If the gate is opened, GFP is expressed whose signal can be measured.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 103
Illegal NheI site found at 284
Illegal NheI site found at 422
Illegal NheI site found at 445 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 224
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1219
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