Coding

Part:BBa_K3279000

Designed by: Zixuan Li   Group: iGEM19_CAU_China   (2019-10-16)
Revision as of 08:16, 16 October 2019 by Registry (Talk | contribs)

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Geranylgeranyl diphosphate synthase (CrtE) from Rhodobacter sphaeroides

This part is a geranylgeranyl diphosphate synthase which transforms (2E,6E)-farnesyl diphosphate into geranylgeranyl diphosphate. It is a crucial enzyme in lycopene synthesis pathway and always works together with phytoene synthase (CrtB) and phytoene desaturase (CrtI). This sequence is optimized for use in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 852
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 531
    Illegal SapI.rc site found at 592


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Categories
Parameters
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