Coding

Part:BBa_K3279002

Designed by: Shujie Liao   Group: iGEM19_CAU_China   (2019-10-14)
Revision as of 05:02, 14 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Phytoene desaturase (CrtI) from Rhodospirillum rubrum

This part is a phytoene desaturase which transforms phytoene into lycopene. It is a crucial enzyme in lycopene synthesis pathway and always works together with geranylgeranyl diphosphate synthase (CrtE) and phytoene synthase (CrtB). This sequence is optimized for use in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 779
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 766
    Illegal NgoMIV site found at 1450
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 325
    Illegal BsaI.rc site found at 820
    Illegal SapI.rc site found at 232


[edit]
Categories
Parameters
None