Coding

Part:BBa_K3286206:Design

Designed by: Ben Kuipers   Group: iGEM19_Wageningen_UR   (2019-10-20)
Revision as of 10:12, 20 October 2019 by Registry (Talk | contribs)

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RpfCch sfGFP fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 757
    Illegal NgoMIV site found at 848
    Illegal NgoMIV site found at 1675
    Illegal NgoMIV site found at 1770
    Illegal AgeI site found at 2326
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To fuse these parts, the stopcodon of RpfCch and the startcodon of the sfGFP were removed and than fused with Golden Gate.


Source

See the BBa_K3286200, BBa_K3286203 parts.

References