Composite

Part:BBa_K3209006

Designed by: SUN YUHAN   Group: iGEM19_JNFLS   (2019-10-16)
Revision as of 10:13, 20 October 2019 by SUNYUHAN (Talk | contribs)


GFP generator driven by Plac, containing LacI gene

We constructed a new GFP generator driven by Plac promoter and contains LacI gene, which can lower significantly the leakage expression. Both LacI and EGFP are linked to the downstream of Plac, which is regulated by LacI inhibitor and IPTG inducer. Using EGFP as a reporter, its fluorescence intensity appears a lower leakage expression. This new GFP generator could be self-regulated because LacI protein can inhibit its self expression, so that no excessive LacI expression which is considered as waste of resources. We detected the response of this generator to different concentration of IPTG, indicating that it could be inhibited by LacI, and induced well by IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2024


[edit]
Categories
Parameters
None