Composite

Part:BBa_K2913005

Designed by: Yaru Gu   Group: iGEM19_NEFU_China   (2019-10-15)
Revision as of 19:30, 15 October 2019 by Registry (Talk | contribs)

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Phuc1, one of the series of Phuc

This composite part consists of Pluc BBa_K2913003, conjugation sequence BBa_K2913002, and a random sequence as long as HucO sequence. Phuc1 includes one hucR binding site inside the Pluc. In the absence of uric acid (or xuanthine), HucR protein binds to Pluc and prevents the binding of RNA polymerase. When uric acid (or xanthine) antagonizes the HucR-Pluc binding, RNA polymerase initiates a transcriptional process of downstream genes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None