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Part:BBa_K3165046

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-16)
Revision as of 00:34, 17 October 2019 by Registry (Talk | contribs)

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i^2mCherry (Ile) Coding Device (under T7 expression)

i^mCherry overcomes the deficiencies in the existing mCherry sequences which undergoes significant truncation in the protein. The single-base mutation at the 48th base causes conversion of the internal start codon into ATC (coding for Ile).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 738
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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