Composite
INT8TLU1

Part:BBa_K3254011

Designed by: Yeqing Zong, Yuan Ruoqi   Group: iGEM19_GENAS_China   (2019-10-16)
Revision as of 06:55, 16 October 2019 by Registry (Talk | contribs)

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Translational Unit for INT8 integrase

This part can be placed downstreamed a prmoter. The leakage transcription of a Ptac promoter on a P15A ori plasmid was sufficient for the recombination of the att sites on low copy vectors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1030
    Illegal AgeI site found at 1427
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination
Parameters
None