Coding

Part:BBa_K3040107

Designed by: Lai, Si-Ying   Group: iGEM19_NTHU_Taiwan   (2019-10-15)
Revision as of 16:08, 16 October 2019 by TALENTTIEN (Talk | contribs)


TesA

Tes A is a heterologous thioesterase, which is able to hydrolyze fatty acyl-CoAs to free fatty acids. Here, we combine Tes A with pFadBA (wild type) together as a new promoter, Tes A pFadBA, to see if the sensitivity range of fatty acid concentration lowers due to the increase of endogenous fatty acid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 981
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 982
    Illegal PstI site found at 996
    Illegal NotI site found at 989
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 982
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 982
    Illegal PstI site found at 996
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None