Composite

Part:BBa_K3088001:Design

Designed by: Syailendra Karuna Sugito, Elvan Wiyarta, Eko Ngadiono, Refael Alfa Budiman, Aditya Parawangsa   Group: iGEM19_UI_Indonesia   (2019-10-07)
Revision as of 09:11, 20 October 2019 by Enga2 (Talk | contribs) (→‎Design Notes)


HBEGF/ENVZ Chimeric Complete


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequencing of this part (BBa_K3088001) combined with part (BBa_K769001) is confirmed and showed as follow:


AAAGATGGACTGACTGAATGCCTCAAATGTTCTTTACGATGCCATTGGGA TATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCT TAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTT ATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAG TGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAA AATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTT CGCTAAGGATGATTTCTGGAAGTGCCATTCCGCCTGACCTAGTTCAAGTG TCCGAGAAGAATTCGCGGCCGCTTCTAGAGAGGTACTGACTCATAAAAAA TTTATTTGCTTTGTGAGCGGATAACAATTATAATAACGATCGCGAAAGAG GAGAAATACGCGATCGAAGATGCGTCGTCTGCGTTTTAGTCCACGCAGCT CGTTTGCACGTACGCTTTTGTTAATCGTTACATTGCTTTTTGCTTCCCTG GTCACCACTTACTTAGTTGTATTAAACTTCGCAATCTTGCCAAGTCTGCA ACAGTTTAACAAGGTATTGGCTTACGAGGTTCGCATGCTTATGACCGACA AACTTCAATTGGAAGACGGGACTCAACTGGTTGTACCACCCGCATTTCGC CGTGAGAAGTACGTTAAGGAATTGCGCGCCCCCAGTTGCATCTGTCACCC CGGATACCATGGAGAGCGCTGCCACGGCTTGTCACTGGTGCCGTTGACAG AGATCCATCAGGGTGACTTTAGCCCGCTTTTCCGTTATACGTTGGCAATT ATGTTACTTGCCATCGGTGGCGCATGGTTATTTATTCGCATTCAGAACCG TCCGTTGGTCGATTTAGAGCACGCTGCACTTCAAGTTGGTAAGGGCATCA TCCCGCCACCATTGCGCGAGTACGGGGCCTCGGAAGTACGTAGTGTTACT CGTGCCTTCAATCATATGGCCGCCGGTGTTAAGCAGTTAGCAGACGACCG CACTCTGTTGATGGCTGGCGGGTCTCATGATTTGCGNACCCCCTGACNCG TATCCGCCTGGCTACTGAAATGATGTCGGAACAGGATGGAACTTGGCCGA GTCCTCAATAAAGACTTGAGGAATGAACGCTTTATTGAACGTTCTCCACT TTTGCTACCGCCCGAAANCCTTTGAANGGGACNNNATGGGGTTGGGGANA ATTTTGCCAAANNGGGTCAACCGGAANNNAAAGGGCTTCCCCNNTTTTGG GGGAAAANNNNNCCCTTTTTTGNNNGGCCCTAAGGTTTAACCNNCCCCCG AAAAGGGGGTAAGGGNCCCNNNCCCCNCCNCCCGTTTTGGNNGGGAGGAC GNCCCCCCCCNA


Figure 1. This the illustration of the plasmid (pSB1C3) containing part BBa K3088001 and BBa_K769001.

Source

This part was obtained by reverse translation from extensive protein modeling of HBEGF/ENVZ. The DNA sequence of both parts were obtained from National Center for Biotechnology Information (NCBI) & UniProt database

References