Composite

Part:BBa_K3088001:Design

Designed by: Syailendra Karuna Sugito, Elvan Wiyarta, Eko Ngadiono, Refael Alfa Budiman, Aditya Parawangsa   Group: iGEM19_UI_Indonesia   (2019-10-07)
Revision as of 09:00, 20 October 2019 by Enga2 (Talk | contribs) (→‎Design Notes)


HBEGF/ENVZ Chimeric Complete


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequencing of this part (BBa_K3088001) combined with part (BBa_K769001) is confirmed and showed as follow:


AAAGATGGACTGACTGAATGCCTCAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAA</p> AAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAAGTGCCATTCCGCCTGACCTAGTTCAAGTGTCCGAGAAGAATTCGCGGCCGCTTCTAGAGAGGTACTGACTCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAACGATCGCGAAAGAGGAGAAATACGCGATCGAAGATGCGTCGTCTGCGTTTTAGTCCACGCAGCTCGTTTGCACGTACGCTTTTGTTAATCGTTACATTGCTTTTTGCTTCCCTGGTCACCACTTACTTAGTTGTATTAAACTTCGCAATCTTGCCAAGTCTGCAACAGTTTAACAAGGTATTGGCTTACGAGGTTCGCATGCTTATGACCGACAAACTTCAATTGGAAGACGGGACTCAACTGGTTGTACCACCCGCATTTCGCCGTGAGAAGTACGTTAAGGAATTGCGCGCCCCCAGTTGCATCTGTCACCCCGGATACCATGGAGAGCGCTGCCACGGCTTGTCACTGGTGCCGTTGACAGAGATCCATCAGGGTGACTTTAGCCCGCTTTTCCGTTATACGTTGGCAATTATGTTACTTGCCATCGGTGGCGCATGGTTATTTATTCGCATTCAGAACCGTCCGTTGGTCGATTTAGAGCACGCTGCACTTCAAGTTGGTAAGGGCATCATCCCGCCACCATTGCGCGAGTACGGGGCCTCGGAAGTACGTAGTGTTACTCGTGCCTTCAATCATATGGCCGCCGGTGTTAAGCAGTTAGCAGACGACCGCACTCTGTTGATGGCTGGCGGGTCTCATGATTTGCGNACCCCCTGACNCGTATCCGCCTGGCTACTGAAATGATGTCGGAACAGGATGGAACTTGGCCGAGTCCTCAATAAAGACTTGAGGAATGAACGCTTTATTGAACGTTCTCCACTTTTGCTACCGCCCGAAANCCTTTGAANGGGACNNNATGGGGTTGGGGANAATTTTGCCAAANNGGGTCAACCGGAANNNAAAGGGCTTCCCCNNTTTTGGGGGAAAANNNNNCCCTTTTTTGNNNGGCCCTAAGGTTTAACCNNCCCCCGAAAAGGGGGTAAGGGNCCCNNNCCCCNCCNCCCGTTTTGGNNGGGAGGACGNCCCCCCCCNA

Figure 1. This the illustration of the plasmid (pSB1C3) containing part BBa K3088001 and BBa_K769001.

Source

This part was obtained by reverse translation from extensive protein modeling of HBEGF/ENVZ. The DNA sequence of both parts were obtained from National Center for Biotechnology Information (NCBI) & UniProt database

===References===