Generator
Part:BBa_K3187039
Designed by: iGEM TU_Darmstadt 2019 Group: iGEM19_TU_Darmstadt (2019-10-13)
tetR/tetA promotor, RBS, mCherry and T7 terminator
This part includes the tetR/tetA promotor + tet operator which can be induced by the addition of anhydrotetracycline (AHT) and a ribosomal binding site.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 76
Illegal PstI site found at 805 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 76
Illegal NheI site found at 810
Illegal PstI site found at 805 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 76
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 76
Illegal PstI site found at 805 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 76
Illegal PstI site found at 805 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |