Composite
phiC31TLU1
Part:BBa_K3254010
Designed by: Yeqing Zong, Li Haoyuan Group: iGEM19_GENAS_China (2019-10-10)
Revision as of 08:15, 16 October 2019 by Zongyeqing (Talk | contribs)
Translational Unit for phiC31 integrase
This part can be placed downstreamed a prmoter. The thermodynamic characteristics of this part were described in the workframe of a previous study(see reference). The RiboJ(BBa_K2615996) part was added before the RBS-coding sequence, so that the modularity and reuseability would be improved.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 150
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1764
Illegal SapI.rc site found at 1455
Illegal SapI.rc site found at 1513
Illegal SapI.rc site found at 1618
[edit]
Categories
Parameters
//function/recombination
None |