Coding
PhASE #2
Part:BBa_K2980014
Designed by: Hongrui Liu Group: iGEM19_Tsinghua (2019-10-16)
(SIM-SUMO)3-SUMO3-mEGFP-Cry2
This part is the fusion of a photo-regulated protein Cry2, a green fluorescence protein mEGFP, and tandem repeats of SIM-SUMO as well as SIM. This part undergoes liquid-liquid phase separation in E. coli, forming green spherical liquid-like puncta. It can be used for light-induced recruitment of other clients into phase. The clients should be connected to the other photo-regulated CIB1 in another vector. Under 488nm laser stimulation, Cry2 and CIB1 will interact with each other, so that the clients will be recruited and enriched in the phase-separated droplets, and further regulation can be performed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 393
Illegal BglII site found at 852
Illegal BamHI site found at 1331 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 277
Illegal AgeI site found at 1006 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 629
Illegal BsaI.rc site found at 38
Illegal SapI.rc site found at 146
[edit]
Categories
Parameters
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