Coding
PhASE #2

Part:BBa_K2980014

Designed by: Hongrui Liu   Group: iGEM19_Tsinghua   (2019-10-16)
Revision as of 16:21, 16 October 2019 by Registry (Talk | contribs)

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(SIM-SUMO)3-SUMO3-mEGFP-Cry2

This part is the fusion of a photo-regulated protein Cry2, a green fluorescence protein mEGFP, and tandem repeats of SIM-SUMO as well as SIM. This part undergoes liquid-liquid phase separation in E. coli, forming green spherical liquid-like puncta. It can be used for light-induced recruitment of other clients into phase. The clients should be connected to the other photo-regulated CIB1 in another vector. Under 488nm laser stimulation, Cry2 and CIB1 will interact with each other, so that the clients will be recruited and enriched in the phase-separated droplets, and further regulation can be performed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 393
    Illegal BglII site found at 852
    Illegal BamHI site found at 1331
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
    Illegal AgeI site found at 1006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 629
    Illegal BsaI.rc site found at 38
    Illegal SapI.rc site found at 146


[edit]
Categories
Parameters
None