Coding
xis

Part:BBa_K112001:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-20)
Revision as of 18:10, 21 October 2008 by Sixpi (Talk | contribs) (→‎Source)

Xis from bacteriophage lambda, assembly standard 21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

PCR bx3/bx2 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112001
----------------------------------------
bx3		Forward biobricking of {<T4 Holin!}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx2		Reverse biobricking of {<T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

References