Coding
xis
Part:BBa_K112001:Design
Designed by: Bing Xia Group: iGEM08_UC_Berkeley (2008-10-20)
Xis from bacteriophage lambda, assembly standard 21
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112001 ---------------------------------------- bx3 Forward biobricking of {<T4 Holin!} ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc bx2 Reverse biobricking of {<T4 Holin!} cgttaGGATCCttatttagcccttcctaatattctggccgcc